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KMID : 0379119950230040332
Korean Journal of Mycology
1995 Volume.23 No. 4 p.332 ~ p.339
Immuno - stimulating Polysaccharides from the Fruiting Bodies of Fomitella fraxinea (1)
ÀÌÀçÈÆ/Lee, Jae Hoon
±èȯ¹¬/ÇÑ»ó¹è/À¯À͵¿/Á¶¼ö¹¬/À¯½ÂÇå/Kim, Hwan Mook/Han, Sang Bae/Yoo, Ick Dong/Cho, Soo Muk/Yu, Seung Hun
Abstract
Water-soluble polysaccharide (FCW) was extracted from the fruiting body of Fomitella fraxinea with neutral sodium chloride solution. The polysaccharide was further fractionated into FCW-I and FCW-II by ion exchange chromatography. The FCW-I and FCW-II were then purified by gel permeation chromatography and named as FCW-Ia and FCW-IIa, respectively. FCW-IIa showed relatively strong immuno-stimulating activity but FCW-Ia did not. By analyses of HPLC and GPC, FCW-Ia and FCW-IIa were identified to be homogeneous and their molecular weights were estimated to be about 15,000 and 8,700, respectively. FCW-Ia consisted of fucose, galactose, and mannose as main sugars and their molar ratio was 19.5 : 63.2 : 25.0. Protein was not detected in FCW-Ia. However, FCW-IIa was composed of glucose, galactose, and mannose at a molar ratio of 1.0 : 0.3 : 0.4 and contained 0.4% protein with a higher amount of glutamic acid. A small amount of uronic acid was detected in both FCW-Ia and FCW-IIa.
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